During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Text Solution
Verified by ExpertsB
Polymerase chain reaction (PCR) involves 3 steps: denaturation, annealing and elongation. The denaturation process requires high temperature of about 
. DNA fragments are joined with the help of DNA ligase enzyme. During annealing, primers bind to the target DNA sequences and initiate polymerisation. This process is performed at 
. Addition of nucleotides to the primer, synthesises a new DNA with the help of enzyme DNA polymerase. This step is called elongation which is performed at 
.
Prepare Smarter with CGP Edu
Get practice questions, solutions, and test series in one place.
Write a Review
Share your experience with this question and solution.
Commentary
Send your comment, doubt, correction, or feedback to admin.
Similar Questions
Explore conceptually related problems